Site-selected incorporation of 5-carboxymethylaminomethyl(- 2-thio)uridine into RNA sequences by phosphoramidite chemistry


5-Carboxymethylaminomethyluridine (cmnm5U) and 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm5U and cmnm5s2U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyl functions, respectively.


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Organic & Biomolecular Chemistry, Vol. 12, Issue 7, pages 1052-1056