In vitro anti-adherence effect of probiotic Lactobacillus strains on human enteropathogens
Data
2017
Autorzy
Tytuł czasopisma
ISSN czasopisma
Tytuł tomu
Wydawca
Lodz University of Technology Press
Wydawnictwa Politechniki Łódzkiej
Wydawnictwa Politechniki Łódzkiej
Abstrakt
Probiotic bacteria possess great potential for producing
antimicrobial substances that inhibit and control pathogenic bacteria in
the human gastrointestinal tract. The aim of this study was to determine the
anti-adherence properties of the probiotic Lactobacillus strains Lb.
rhamnosus ŁOCK 0900, Lb. rhamnosus ŁOCK 0908, and Lb. casei ŁOCK
0919 (individually and in a 1:1:2 mixture) against the reference pathogens
Clostridium difficile (ATCC 9689), Enterococcus faecalis (ATCC 29212),
Listeria monocytogenes (ATCC 19115), and Staphylococcus aureus (ATCC
6538) using the Caco-2 human colon adenocarcinoma cell line. The
mixture of probiotic strains inhibited the adherence of all pathogens, from
10.2% for E. faecalis ATCC 29212 to 97.2% for L. monocytogenes ATCC
19115. Of all the tested probiotic strains, Lb. casei ŁOCK 0919 reduced
the adherence of S. aureus ATCC 6538 to the greatest extent (by 45.9%).
These results suggest that adherence inhibition may involve competition for
eukaryotic cell receptors and probiotic bacteria could protect the host cells
from pathogen colonization and disease.
Opis
Słowa kluczowe
Adherence, intestinal microbiota, pathogens, inhibition, Caco-2 cells, przyczepność, mikroflora, czynniki chorobotwórcze, wstrzymywanie
Cytowanie
Nowak, A., & Motyl, I. (2017). In vitro anti-adherence effect of probiotic Lactobacillus strains on human enteropathogens. Biotechnology and Food Science, 81(2), 103-112. https://doi.org/10.34658/bfs.2017.81.2.103-112