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Pozycja A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis(2017) Boncler, Magdalena; Golanski, Jacek; Lukasiak, Magdalena; Redzynia, Malgorzata; Dastych, Jaroslaw; Watala, Cezary; Medical University of Lodz. Department of Haemostasis and Haemostatic Disorders.; Proteon Pharmaceuticals SA; Lodz University of Technology. Faculty of Biotechnology and Food Sciences. Institute of Technical Biochemistry.; Polish Academy of Sciences. Institute of Medical Biology. Laboratory of Cellular Immunology.The toxicity of in vitro tested compounds is usually evaluated based on EC50 values calculated from dose-response curves. However, there is a large group of compounds for which a standard four-parametric sigmoid curve fitting may be inappropriate for estimating EC50. In the present study, 22 polyphenol-rich compounds were prioritized from the least to the most toxic based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The studied compounds were ranked across three key cell indicators (mitochondrial membrane potential, cell membrane integrity and nuclear size) in a panel of five cell lines (HepG2, Caco-2, A549, HMEC-1, and 3T3), using a high-content screening (HCS) assay. Regarding AUOC score values, naringin (negative control) was the least toxic phenolic compound. Aronox, spent hop extract and kale leaf extract had very low cytotoxicity with regard to mitochondrial membrane potential and cell membrane integrity, as well as nuclear morphology (nuclear area). Kaempferol (positive control) exerted strong cytotoxic effects on the mitochondrial and nuclear compartments. Extracts from buckthorn bark, walnut husk and hollyhock flower were highly cytotoxic with regard to the mitochondrion and cell membrane, but not the nucleus. We propose an alternative algorithm for the screening of a large number of agents and for identifying those with adverse cellular effects at an early stage of drug discovery, using high content screening analysis. This approach should be recommended for series of compounds producing a non-sigmoidal cell response, and for agents with unknown toxicity or mechanisms of action.Pozycja A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis(2017) Boncler, Magdalena; Golanski, Jacek; Lukasiak, Magdalena; Redzynia, Malgorzata; Dastych, Jaroslaw; Watala, Cezary; Medical University of Lodz. Department of Haemostasis and Haemostatic Disorders.; Proteon Pharmaceuticals SA; Lodz University of Technology. Faculty of Biotechnology and Food Sciences. Institute of Technical Biochemistry.; Polish Academy of Sciences. Institute of Medical Biology. Laboratory of Cellular Immunology.The toxicity of in vitro tested compounds is usually evaluated based on EC50 values calculated from dose-response curves. However, there is a large group of compounds for which a standard four-parametric sigmoid curve fitting may be inappropriate for estimating EC50. In the present study, 22 polyphenol-rich compounds were prioritized from the least to the most toxic based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The studied compounds were ranked across three key cell indicators (mitochondrial membrane potential, cell membrane integrity and nuclear size) in a panel of five cell lines (HepG2, Caco-2, A549, HMEC-1, and 3T3), using a high-content screening (HCS) assay. Regarding AUOC score values, naringin (negative control) was the least toxic phenolic compound. Aronox, spent hop extract and kale leaf extract had very low cytotoxicity with regard to mitochondrial membrane potential and cell membrane integrity, as well as nuclear morphology (nuclear area). Kaempferol (positive control) exerted strong cytotoxic effects on the mitochondrial and nuclear compartments. Extracts from buckthorn bark, walnut husk and hollyhock flower were highly cytotoxic with regard to the mitochondrion and cell membrane, but not the nucleus. We propose an alternative algorithm for the screening of a large number of agents and for identifying those with adverse cellular effects at an early stage of drug discovery, using high content screening analysis. This approach should be recommended for series of compounds producing a non-sigmoidal cell response, and for agents with unknown toxicity or mechanisms of action.Pozycja Lotne pochodne furanu(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Balawejder, Maciej; Kula, JózefPozycja Analiza chromatograficzna i izolacja składników olejków eterycznych z liści i drewna Calocedrus decurrens (Torr.) Florin(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Włodarczyk, Maciej; Kalemba, Danuta; Szumny, Antoni; Cisowski, WojciechPozycja Wpływ metody wydzielania związków lotnych na efektywność ich oznaczania w surowcu roślinnym(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Wajs, Anna; Kalemba, DanutaPozycja Rozwój gospodarki opartej na wiedzy – źródła finansowania postępu technologicznego i innowacyjnego przedsiębiorstw(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Wojtczak, MaciejPozycja Substancje zapachowe i kosmetyczne biogenerowane w skali przemysłowej(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Kula, Józef; Sikora, MagdalenaPozycja Synteza nienasyconych alkoholi, potencjalnych związków zapachowych, w reakcji przeniesienia wodoru do α,β-nienasyconych związków karbonylowych(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Gliński, Marek; Ulkowska, Urszula; Gibka, JuliaPozycja Synteza i właściwości zapachowe tlenowych pochodnych1,5,5-trimetylocykloheksenu(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Wińska, Katarzyna; Gibka, Julia; Sikora, Małgorzata; Kula, Józef; Wawrzeńczyk, CzesławPozycja Mikroenkapsulacja olejków eterycznych w warunkach suszenia rozpryskowego(Institute of General Food Chemistry. Faculty of Biotechnology and Food Sciences., 2007) Adamiec, Janusz; Kalemba, Danuta
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