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Pozycja Safety of workers exposed to harmful airborne bioaerosols – legal status and innovations(Wydawnictwo Politechniki Łódzkiej, 2017) Majchrzycka, Katarzyna; Okrasa, Małgorzata; Szulc, Justyna; Gutarowska, BeataFiltering respiratory protective equipment is commonly used for protection against bioaerosols in industrial workplaces. However, the EU legislation does not specify the requirements for its antimicrobial properties. The result is that in the market there is no equipment available that would ensure complete safety of workers exposed to inhalation of harmful bioaerosols. This is particularly important in the case of equipment intended for multiple and long-term use. The aim of this study was to develop an innovative filtering half mask for the protection of respiratory tract that would have confirmed antimicrobial properties. The half mask was tested for compliance with EN 149:2001+A1:2009, and the survival rate of microorganisms in nonwovens was determined (E. coli bacteria and A. niger moulds). The reduction in the number of E. coli bacteria was 98.69% and the reduction in the number of A. niger moulds was 67.68% after 32 h of storage under conditions simulating the conditions of use of the equipment. It was found that the biocidal activity of the half mask is sufficient to ensure a significant reduction in the number of microorganisms during its long-term use in the workplace. This in turn means that continuous disinfection of respiratory protective equipment would be maintained even without employees’ intervention, which is not possible in case of standard respiratory protective equipment.Pozycja Ocena zanieczyszczenia mikrobiologicznego na stanowiskach pracy w garbarniach(Polish Society of Occupational Medicine, 2014) Skóra, Justyna; Gutarowska, Beata; Stępień, Łukasz; Otlewska, Anna; Pielech-Przybylska, KatarzynaBackground: Due to their animal material processing, tannery workers may be exposed to biological agents. The aim of the study was the microbial contamination assessment of tanneries with different production specifications. Health risk was estimated based on particle size distribution. Moreover, indicators of microbial contamination of tanneries were selected. Materials and Methods: The studies were conducted in 2 types of tanneries – processing raw hides and producing chrome-tanned leather. Air was sampled with MAS-100 Eco Air Sampler, leathers using RODAC Envirocheck® contact plates and swab method, microbial numbers were determined by a culture method. For the bioaerosols size distribution analysis, a six-stage Andersen sampler was used; identification was performed using microscopy and biochemical methods. Microbial contamination was identified by 16S RNA and ITS1/2 rDNA analysis for bacteria and fungi respectively. Results: The microbial number in the air ranged between 1.2×103 and 3.7×103 CFU/m3. While on the leather, it ranged between 7.6×101 and 5.5×105 CFU/100 cm2. Bacteria dominated in the tanneries (air: 51–92%, leathers: 60–100%). Results indicate that potential health risks arise from the fungal small bioaerosol particles presence (0.65–2.1 μm). Eleven indicator microorganisms were determined: B. pumilus, B. subtilis, B. cereus, C. lubricantis, C. cladosporioides, P. commune, P. echinulatum, P. chrysogenum, P. crustosum C. parapsilosis and C. albidus. Conclusions: Microbial contamination evaluation in the tanneries showed the increased bacteria and fungi number in the air in relation to the outdoor air, which indicates an occupational inhalation risk to workers. The designated indicators of microbial contamination in the tanneries are associated with their specific and potentially pathogenic working environment.