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Stały URI dla kolekcjihttp://hdl.handle.net/11652/147

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20071

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Autorsearch.filters.applied.operator.equals: search.filters.author.Jabłońska, JoannaTematsearch.filters.applied.operator.equals: search.filters.subject.intensywność fluorescencji FAD

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    The thermal scanning fluorescence study on the conformational stability of glucose oxidase (GOD)
    (Wydawnictwo Politechniki Łódzkiej, 2007) Kowalska, Agnieszka; Gyugos, Monika; Szego, Dora; Pineda, Adrianna Lopez; Ayala, Diana; Xu, Yuan; Hughes, Nichola; Tito, Antonio; Jabłońska, Joanna
    In this study, the temperature-induced unfolding process of glucose oxidase (GOD) from Aspergillus niger has been investigated by monitoring fluorescence intensity of FAD, which dissociates from the enzyme during denaturation. The temperature, as well as Gibbs free energy of the unfolding process of GOD have been determined. Additionally, the effect of glucose on the flavoprotein thermostability has also been studied. The obtained results suggest that thermal scanning fluorescence method is a sensitive, fast and simple method for recording conformational changes associated with the temperature-induced unfolding process of GOD.