Artykuły (WBiNoŻ)

Stały URI dla kolekcjihttp://hdl.handle.net/11652/147

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  • Pozycja
    Determination of nickel in tea by using dimethylglyoxime method
    (Wydawnictwo Politechniki Łódzkiej, 2010) Sykuła, Anna; Turek, Monika; Mohit, Philip Mathew; Patai, Ferenc; Horvat, Martina; Jabłońska, Joanna
    Tea is one of the most popular beverages in the world. Recent studies have shown that it contains concentrations of heavy metals such as nickel. Heavy metals are known to be toxic and there have been many studies on their toxicity such as lead and cadmium, however researchers are now starting to explore the nature of the toxicity of nickel. A common and well-known method of spectrophotometric determination of nickel by dimethylglyoxime reagent was used. The presented paper, due to the increasing concerns surrounding the harmful effects of nickel compounds, aims at testing and demonstration of the effectiveness of the mentioned method.
  • Pozycja
    The study of interactions between Diclofenac and Bovine Serum Albumin (BSA)
    (Wydawnictwo Politechniki Łódzkiej, 2008) Jabłońska, Joanna; Casares, Daniela; Funfer, Johanna; Habenschuss, Thomas; Jimenez, Gabriela; Klemm, Anna Lena; Machado, Antonio Magela Junior; Miloshevska, Sandra; Papista, Ivana; Urlich-Lynge, Sofie Louise
    Investigation of the interactions between diclofenac and BSA was performed using UV-Vis absorption and fluorescence methods. There was a quenching effect found between these two molecules in the fluorescence measurement methods; steady-state and time-resolved. The fluorescence decay time enables to determine the quenching mechanism and thus conclude that the complex is formed in ground-state but also collisional quenching - when the excited-state fluorophore of BSA is deactivated - occurs. Computer modeling was applied to observe and optimize the formation of the complex between N-acetyl-L-tryptophamide and diclofenac.
  • Pozycja
    The thermal scanning fluorescence study on the conformational stability of glucose oxidase (GOD)
    (Wydawnictwo Politechniki Łódzkiej, 2007) Kowalska, Agnieszka; Gyugos, Monika; Szego, Dora; Pineda, Adrianna Lopez; Ayala, Diana; Xu, Yuan; Hughes, Nichola; Tito, Antonio; Jabłońska, Joanna
    In this study, the temperature-induced unfolding process of glucose oxidase (GOD) from Aspergillus niger has been investigated by monitoring fluorescence intensity of FAD, which dissociates from the enzyme during denaturation. The temperature, as well as Gibbs free energy of the unfolding process of GOD have been determined. Additionally, the effect of glucose on the flavoprotein thermostability has also been studied. The obtained results suggest that thermal scanning fluorescence method is a sensitive, fast and simple method for recording conformational changes associated with the temperature-induced unfolding process of GOD.