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Antimicrobial Activity and Filtration Effectiveness of Nonwovens with Sanitized for Respiratory Protective Equipment
(Institute of Biopolymers and Chemical Fibres, 2014) Gutarowska, Beata; Skóra, Justyna; Nowak, Ewelina; Łysiak, Izabela; Wdówka, Malwina
The objective of the study was to optimise the production of bioactive filtration nonwovens with Sanitized® T 99-19, containing quaternary ammonium salts, by evaluating different production technologies (melt-blowing, needle punching), methods of biocide incorporation (bath, spraying), biocide concentration, and conditioning. The antimicrobial activity of nonwovens was tested against different microorganisms, from culture collection and workplaces, using the method AATCC 100. It was shown that the biological efficiency of nonwovens rose when the concentration of Sanitized was increased from 0.7% to 2%. Furthermore, higher biological activity was found in nonwovens subjected to a bath than in those which underwent spraying. The conditioning process did not significanty affect the antimicrobial activity of the nonwovens tested. As compared to melt-blown nonwovens, the needled variety were more efficient against both collection strains and those isolated from workplaces. Thus both types of nonwovens may be used for the production of bioactive halfmasks protecting the respiratory tract of workers exposed to microorganisms
Ocena zanieczyszczenia mikrobiologicznego na stanowiskach pracy w garbarniach
(Polish Society of Occupational Medicine, 2014) Skóra, Justyna; Gutarowska, Beata; Stępień, Łukasz; Otlewska, Anna; Pielech-Przybylska, Katarzyna
Background: Due to their animal material processing, tannery workers may be exposed to biological agents. The aim of the study was the microbial contamination assessment of tanneries with different production specifications. Health risk was estimated based on particle size distribution. Moreover, indicators of microbial contamination of tanneries were selected. Materials and Methods: The studies were conducted in 2 types of tanneries – processing raw hides and producing chrome-tanned leather. Air was sampled with MAS-100 Eco Air Sampler, leathers using RODAC Envirocheck® contact plates and swab method, microbial numbers were determined by a culture method. For the bioaerosols size distribution analysis, a six-stage Andersen sampler was used; identification was performed using microscopy and biochemical methods. Microbial contamination was identified by 16S RNA and ITS1/2 rDNA analysis for bacteria and fungi respectively. Results: The microbial number in the air ranged between 1.2×103 and 3.7×103 CFU/m3. While on the leather, it ranged between 7.6×101 and 5.5×105 CFU/100 cm2. Bacteria dominated in the tanneries (air: 51–92%, leathers: 60–100%). Results indicate that potential health risks arise from the fungal small bioaerosol particles presence (0.65–2.1 μm). Eleven indicator microorganisms were determined: B. pumilus, B. subtilis, B. cereus, C. lubricantis, C. cladosporioides, P. commune, P. echinulatum, P. chrysogenum, P. crustosum C. parapsilosis and C. albidus. Conclusions: Microbial contamination evaluation in the tanneries showed the increased bacteria and fungi number in the air in relation to the outdoor air, which indicates an occupational inhalation risk to workers. The designated indicators of microbial contamination in the tanneries are associated with their specific and potentially pathogenic working environment.