Przeglądaj {{ collection }} wg Autor "Koziołkiewicz, Maria"

Teraz wyświetlane 1 - 2 z 2

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors
(Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, 2014) Szewczyk, Karolina; Lewandowska, Urszula; Owczarek, Katarzyna; Sosnowska, Dorota; Gorlach, Sylwia; Koziołkiewicz, Maria; Hrabec, Zbigniew; Hrabec, Elżbieta
Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid - ᵧ-linolenic acid (GLA) - but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the influence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography. The results revealed that the examined polyphenol extract had little influence on Caco-2 proliferation, but effectively in a time- and dose-dependent manner inhibited MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF-α and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 μmol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.
Polyphenols Extracts as Inhibitors of Glycolytic Enzymes
(Lodz University of Technology Press, 2015) Majewska, Iwona; Koziołkiewicz, Maria
An increasing number of people diagnosed with diabetes is connected with changes in life style and the carbohydrate-rich diet. Despite the existence of a pool of antidiabetic drugs, there is a need to find a new pharmaceuticals including those derived from plant materials. Polyphenols can be an interesting alternative of antihyperglycemic agents.