• polski
    • English
Lodz University of Technology Repository
TUL Repository
  • English 
    • polski
    • English
  • Login
View Item 
  •   Home
  • Wydział Biotechnologii i Nauk o Żywności / Faculty of Biotechnology and Food Sciences / W5
  • Inne (WBiNoŻ)
  • View Item
  •   Home
  • Wydział Biotechnologii i Nauk o Żywności / Faculty of Biotechnology and Food Sciences / W5
  • Inne (WBiNoŻ)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis

Thumbnail
View/Open
Approach_assessment_toxicity_polyphenol_rich_compounds_Boncler_2017.pdf (3.722Mb)
Date
2017
Author
Boncler, Magdalena
Golanski, Jacek
Lukasiak, Magdalena
Redzynia, Malgorzata
Dastych, Jaroslaw
Watala, Cezary
Metadata
Show full item record
Abstract
The toxicity of in vitro tested compounds is usually evaluated based on EC50 values calculated from dose-response curves. However, there is a large group of compounds for which a standard four-parametric sigmoid curve fitting may be inappropriate for estimating EC50. In the present study, 22 polyphenol-rich compounds were prioritized from the least to the most toxic based on the total area under and over the dose-response curves (AUOC) in relation to baselines. The studied compounds were ranked across three key cell indicators (mitochondrial membrane potential, cell membrane integrity and nuclear size) in a panel of five cell lines (HepG2, Caco-2, A549, HMEC-1, and 3T3), using a high-content screening (HCS) assay. Regarding AUOC score values, naringin (negative control) was the least toxic phenolic compound. Aronox, spent hop extract and kale leaf extract had very low cytotoxicity with regard to mitochondrial membrane potential and cell membrane integrity, as well as nuclear morphology (nuclear area). Kaempferol (positive control) exerted strong cytotoxic effects on the mitochondrial and nuclear compartments. Extracts from buckthorn bark, walnut husk and hollyhock flower were highly cytotoxic with regard to the mitochondrion and cell membrane, but not the nucleus. We propose an alternative algorithm for the screening of a large number of agents and for identifying those with adverse cellular effects at an early stage of drug discovery, using high content screening analysis. This approach should be recommended for series of compounds producing a non-sigmoidal cell response, and for agents with unknown toxicity or mechanisms of action.
URI
http://hdl.handle.net/11652/1482
http://hdl.handle.net/11652/1722
Collections
  • Inne (WBiNoŻ) [30]

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

DSpace software copyright © 2002-2016  DuraSpace
Contact Us | Send Feedback
Theme by 
Atmire NV