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Site-selected incorporation of 5-carboxymethylaminomethyl(- 2-thio)uridine into RNA sequences by phosphoramidite chemistry

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Date
2014
Author
Leszczyńska, Grażyna
Pięta, Jakub
Woźniak, Karolina
Małkiewicz, Andrzej
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Abstract
5-Carboxymethylaminomethyluridine (cmnm5U) and 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm5U and cmnm5s2U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyl functions, respectively.
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http://hdl.handle.net/11652/1126
http://pubs.rsc.org/en/content/articlelanding/2014/ob/c3ob42302f#!divAbstract
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  • Artykuły (WCh) [128]

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