Leszczyńska, GrażynaPięta, JakubWoźniak, KarolinaMałkiewicz, Andrzej2016-02-082016-02-082014Organic & Biomolecular Chemistry, Vol. 12, Issue 7, pages 1052-1056http://hdl.handle.net/11652/1126http://pubs.rsc.org/en/content/articlelanding/2014/ob/c3ob42302f#!divAbstract5-Carboxymethylaminomethyluridine (cmnm5U) and 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm5U and cmnm5s2U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyl functions, respectively.enArtykuł