Nowak, AdrianaMotyl, Ilona2017-09-042017-09-042017Biotechnology and Food Science 2017, Vol.81 No.2, p.103-1122084-0136Brak expIDhttp://www.bfs.p.lodz.plProbiotic bacteria possess great potential for producing antimicrobial substances that inhibit and control pathogenic bacteria in the human gastrointestinal tract. The aim of this study was to determine the anti-adherence properties of the probiotic Lactobacillus strains Lb. rhamnosus ŁOCK 0900, Lb. rhamnosus ŁOCK 0908, and Lb. casei ŁOCK 0919 (individually and in a 1:1:2 mixture) against the reference pathogens Clostridium difficile (ATCC 9689), Enterococcus faecalis (ATCC 29212), Listeria monocytogenes (ATCC 19115), and Staphylococcus aureus (ATCC 6538) using the Caco-2 human colon adenocarcinoma cell line. The mixture of probiotic strains inhibited the adherence of all pathogens, from 10.2% for E. faecalis ATCC 29212 to 97.2% for L. monocytogenes ATCC 19115. Of all the tested probiotic strains, Lb. casei ŁOCK 0919 reduced the adherence of S. aureus ATCC 6538 to the greatest extent (by 45.9%). These results suggest that adherence inhibition may involve competition for eukaryotic cell receptors and probiotic bacteria could protect the host cells from pathogen colonization and disease.application/pdfplAdherenceintestinal microbiotapathogensinhibitionCaco-2 cellsprzyczepnośćmikrofloraczynniki chorobotwórczewstrzymywanieArtykuł