Nowak, AdrianaMotyl, Ilona2017-09-042017-09-042017Nowak, A., & Motyl, I. (2017). In vitro anti-adherence effect of probiotic Lactobacillus strains on human enteropathogens. Biotechnology and Food Science, 81(2), 103-112. https://doi.org/10.34658/bfs.2017.81.2.103-1122084-01362299-6818 (online version)Brak expIDhttp://www.bfs.p.lodz.plhttps://doi.org/10.34658/bfs.2017.81.2.103-112Probiotic bacteria possess great potential for producing antimicrobial substances that inhibit and control pathogenic bacteria in the human gastrointestinal tract. The aim of this study was to determine the anti-adherence properties of the probiotic Lactobacillus strains Lb. rhamnosus ŁOCK 0900, Lb. rhamnosus ŁOCK 0908, and Lb. casei ŁOCK 0919 (individually and in a 1:1:2 mixture) against the reference pathogens Clostridium difficile (ATCC 9689), Enterococcus faecalis (ATCC 29212), Listeria monocytogenes (ATCC 19115), and Staphylococcus aureus (ATCC 6538) using the Caco-2 human colon adenocarcinoma cell line. The mixture of probiotic strains inhibited the adherence of all pathogens, from 10.2% for E. faecalis ATCC 29212 to 97.2% for L. monocytogenes ATCC 19115. Of all the tested probiotic strains, Lb. casei ŁOCK 0919 reduced the adherence of S. aureus ATCC 6538 to the greatest extent (by 45.9%). These results suggest that adherence inhibition may involve competition for eukaryotic cell receptors and probiotic bacteria could protect the host cells from pathogen colonization and disease.application/pdfenFair use conditionDla wszystkich w zakresie dozwolonego użytkuAdherenceintestinal microbiotapathogensinhibitionCaco-2 cellsprzyczepnośćmikrofloraczynniki chorobotwórczewstrzymywanieArtykułLUT LicenseLicencja PŁ10.34658/bfs.2017.81.2.103-112