Biotechnology and Food Science

Stały URI zbioruhttp://hdl.handle.net/11652/3940

Biotechnology and Food Science publikuje artykuły oryginalne i przeglądowe dotyczące wszystkich aspektów biotechnologii i żywności oraz dziedzin pokrewnych.Czasopismo jest miejscem wymiany informacji w następujących dziedzinach:biotechnologia, chemia, technologia i toksykologia żywności, chemia i biotechnologia środowiska, chemia nieorganiczna, organiczna i fizyczna, biochemia, enzymologia, mikrobiologia, chemia medyczna, inżynieria chemiczna korozja mikrobiologiczna. Biotechnology and Food Science jest czasopismem ogólnie dostępnym, ukazuje się dwa razy w roku w wersji drukowanej i on-line. Pełne teksty artykułów są dostępne (www.bfs.p.lodz.pl). Wszystkie prace są recenzowane i oceniane pod względem jakości i oryginalności.

Czasopismo wydawane jest od 1955 roku, najpierw jako Zeszyty Naukowe Politechniki Łódzkiej. Chemia Spożywcza (1955-1979), następnie jako Zeszyty Naukowe Politechniki Łódzkiej. Technologia i Chemia Spożywcza (1980-1997) i Zeszyty Naukowe Politechniki Łódzkiej. Chemia Spożywcza i Biotechnologia (1998-2010).

ISSN 2299-6818 Częstotliwość wydawania: półrocznik (wersja drukowana i online) Wersja pierwotna: druk Język: angielski

Redaktor naczelny: Małgorzata Piotrowska tel: +48 42-631-34-70, e-mail: malgorzata.piotrowska@p.lodz.pl Adres redakcji: Wydział Biotechnologii i Nauk o Żywności Politechniki Łódzkiej Stefanowskiego 4/10, 90-924 Łódź tel.: 42-631-34-10, 42-636-28-60 fax: +48 42-636-28-60 e-mail: bfs@info.p.lodz.pl Wydawca: Wydawnictwo Politechniki Łódzkiej Wólczańska 223, 90-924 Łódź phone/fax: +48 42 631 29 52 www.wydawnictwa.p.lodz.pl

DOI: https://doi.org/10.34658/bfs/ Punkty MNiSW 2019: 5

Indeksacja: BazTech Agro Index Copernicus - ICI Journals Master List Dziedziny: rolnictwo, biotechnologia i mikrobiologia stosowana, chemia, nauka i technologia żywności

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Zinc and nano-ZnO – influence on living organisms
(Lodz University of Technology Press, 2017) Mielcarz-Skalska, Lidia; Smolińska, Beata
The use of zinc is very common in many industries. The multitude of advantages, and capabilities make use of different forms of penetration of zinc into the environment. Due to its ability to migrate, zinc is also accumulated in living organisms. Zn is an essential trace element but both the deficiency or excess are extremely dangerous for living organisms. Nowadays nanozinc has been increasingly used. Nanozinc is a component of daily used products, like plastics, ceramics, glass, cement, rubber, foods and many others. This leads to spreading nanozinc the environment with the possibility of its penetration into living organisms. This article is a review of the impact of zinc and zinc nanoparticles on a variety of organisms: ranging from microorganisms through plants and animals, to people.
A comparison of two enzymatic methods of clinical dextran production
(Wydawnictwo Politechniki Łódzkiej, 2017) Sikora, Barbara; Kubik, Celina; Bielecki, Stanisław
The aim of this study was to evaluate and compare of the two enzymatic methods of clinical dextran production were compared. The reactions were performed at 30°C and pH 5.4 in solutions containing different amounts of sucrose, using dextransucrase (DS, in the presence of dextranase (D) (method 1) or acceptor dextrans (method 2). The activity of Leuconostoc mesenteroides L dextransucrase (DS), which converts sucrose to dextran, was 0.4 U ml-1 in both the methods. As much as 53-56% of clinical dextran fractions were obtained for 28 h from 10% sucrose solutions, which contained 1.5% or 2.5% acceptor dextrans with molecular mass of 10 and 15 kDa, respectively. Approximately 50% of these fractions was obtained (also in 28 h) from 10% sucrose solutions by using 0.004 U ml-1 of DN, added to reaction mixtures 5 h later than Our experiments indicate that the clinical dextran can be efficiently produced by using both the compared methods, which employ either acceptor dextrans with definite molecular mass, or the dextranase. Because consumption of the latter enzyme is rather small, and it is easily available, thus this method should be attractive for clinical dextran manufacturers.
Production of co-immobilized dextransucrase and dextranase preparations and their application in isomalto-oligosaccharides synthesis
(Wydawnictwo Politechniki Łódzkiej, 2017) Sikora, Barbara; Kubik, Celina; Bielecki, Stanisław
Dextransucrase (DS) from Leuconostoc mesenteroides and dextranase (DN) from Penicillium funiculosum were co-immobilized by entrapment in calcium alginate and used to produce isomaltooligosaccharides (IMOs) from sucrose. DS convert sucrose into dextran, which is the substrate for DN, so that IMOs are products of dextran hydrolysis. Before the co-immobilization DS was cross-linked with glutardialdehyde (GA), while DN was adsorbed on hydroxyapatite (HAp). Cross-linking was essential for the stability of DS and pre-immobilization of DN to prevent enzyme from leaking out of the alginate beads. Operational stability of co-immobilized preparations of DS and DN was estimated based on amounts of isomaltose and isomaltotriose formed during successive 24h processes of IMOs synthesis, carried out at 30oC, pH 5.4 and 200 rpm in 10% (w/v) sucrose solutions. Preparation characterized by the initial DS/DN activities ratio of 1/14 was found to maintain these activities at least 100 h of IMOs synthesis (5 repeated batch reaction).
In vitro anti-adherence effect of probiotic Lactobacillus strains on human enteropathogens
(Lodz University of Technology Press, 2017) Nowak, Adriana; Motyl, Ilona
Probiotic bacteria possess great potential for producing antimicrobial substances that inhibit and control pathogenic bacteria in the human gastrointestinal tract. The aim of this study was to determine the anti-adherence properties of the probiotic Lactobacillus strains Lb. rhamnosus ŁOCK 0900, Lb. rhamnosus ŁOCK 0908, and Lb. casei ŁOCK 0919 (individually and in a 1:1:2 mixture) against the reference pathogens Clostridium difficile (ATCC 9689), Enterococcus faecalis (ATCC 29212), Listeria monocytogenes (ATCC 19115), and Staphylococcus aureus (ATCC 6538) using the Caco-2 human colon adenocarcinoma cell line. The mixture of probiotic strains inhibited the adherence of all pathogens, from 10.2% for E. faecalis ATCC 29212 to 97.2% for L. monocytogenes ATCC 19115. Of all the tested probiotic strains, Lb. casei ŁOCK 0919 reduced the adherence of S. aureus ATCC 6538 to the greatest extent (by 45.9%). These results suggest that adherence inhibition may involve competition for eukaryotic cell receptors and probiotic bacteria could protect the host cells from pathogen colonization and disease.
Safety of workers exposed to harmful airborne bioaerosols – legal status and innovations
(Wydawnictwo Politechniki Łódzkiej, 2017) Majchrzycka, Katarzyna; Okrasa, Małgorzata; Szulc, Justyna; Gutarowska, Beata
Filtering respiratory protective equipment is commonly used for protection against bioaerosols in industrial workplaces. However, the EU legislation does not specify the requirements for its antimicrobial properties. The result is that in the market there is no equipment available that would ensure complete safety of workers exposed to inhalation of harmful bioaerosols. This is particularly important in the case of equipment intended for multiple and long-term use. The aim of this study was to develop an innovative filtering half mask for the protection of respiratory tract that would have confirmed antimicrobial properties. The half mask was tested for compliance with EN 149:2001+A1:2009, and the survival rate of microorganisms in nonwovens was determined (E. coli bacteria and A. niger moulds). The reduction in the number of E. coli bacteria was 98.69% and the reduction in the number of A. niger moulds was 67.68% after 32 h of storage under conditions simulating the conditions of use of the equipment. It was found that the biocidal activity of the half mask is sufficient to ensure a significant reduction in the number of microorganisms during its long-term use in the workplace. This in turn means that continuous disinfection of respiratory protective equipment would be maintained even without employees’ intervention, which is not possible in case of standard respiratory protective equipment.
5-hydroxymethylfurfural content in selected gluten- and gluten-free cereal food products
(Lodz University of Technology Press, 2017) Mańkowska, Dorota; Majak, Iwona; Bartos, Adrian; Słowianek, Marta; Łącka, Agata; Leszczyńska, Joanna
5-Hydroxymethylfurfural (HMF) was determined with HPLC method in forty one food samples, including gluten-free, breakfast cereals and bakery products. The highest concentration of HMF was found in wheat bread with cranberries (210 mg kg-1) and in breakfast cereals – honey wheat loops (85.099 mg kg-1). In contrast, wholegrain oatmeal and gluten-free sponge cakes had the lowest HMF level of all tested samples, below the detection limit and 0.485 mg kg-1, respectively. In most cases, lack of gluten coincided with the lowest HMF content readings (average 8.488 mg kg-1). The impact of the type of sugar, especially glucose, on the HMF concentration in food is apparent. Sweetened breakfast cereals, with the average content of HMF at 25.55 mg kg-1, took lead over dietary products (8.488 mg kg-1) and bakery products (18.395 mg kg-1), with the exception for wheat bread with cranberries. These cereals contained glucose or glucose-fructose syrup.
Plant biomass degradation supported by non-enzymatic proteins
(Lodz University of Technology Press, 2017) Madaj, Rafał; Kalinowska, Halina; Sobiecka, Elżbieta
Lignocellulosic biomass, rich in potential carbon sources and value added products, has been intensively investigated in scope of its costefficient and effective decomposition. Many methods were developed, physicochemical or biological. Nevertheless, they are either expensive, inefficient or pose threat to the environment. Recently discovered proteins, lacking any hydrolytic activity, can be a key to solve problems associated with a slow process of enzymatical, eco-friendly degradation. These proteins belong to three related groups – swollenins, expansins and loosenins. Using different molecular mechanisms, they disrupt hydrogen bonds within cellulose chains, enabling enzymes to perform hydrolysis leading to decomposition of lignocellulosic complex.
Extremophilic and modified aminotransferases as a versatile tool for the synthesis of optically pure building blocks for pharmaceutical industry
(Lodz University of Technology Press, 2017) Szmejda, Klaudia; Florczak, Tomasz; Jodłowska, Iga; Turkiewicz, Marianna
Considerable progress has been made in the past few years with industrial use of essential key intermediates for chemical and pharmaceutical industry. The increasing demand for obtaining chiral drugs in enantiomerically pure form makes it necessary to search for novel biocatalysts useful in the synthesis of amino acids, chiral amines, amino sugars and alcohols. According to the reaction mechanism, aminotransferases (ATs) have useful applications because of their capability of transfer of an amino group from a donor substrate to an acceptor, thus resulting in the synthesis of a wide variety of building blocks. This article reviews current biocatalytic approaches using microbial ATs in the synthesis of optically active products. Focus is also put on the engineering of ATs and their limitations in the industrial applications. Moreover this review covers biocatalytic approaches using ATs isolated from extreme environments.
Laccases – enzymes with an unlimited potential
(Lodz University of Technology Press, 2017) Szczęsna-Antczak, Mirosława; Benedykt Kaczmarek, Michał; Kwiatos, Natalia; Bielecki, Stanisław
Laccases (EC 1.10.3.2) are among the few enzymes, the history of which dates back to the 19th century. These oxidoreductases are present in almost all known fungi, some species of higher plants and insects. Moreover, in recent years, these enzymes have also been found in some bacterial organisms. Due to their significant properties and structure of the catalytic centre, laccases have been classified as the multicopper oxidases (MCOs). These enzymes are able to catalyse the oxidation of phenolic and non-phenolic compounds, with the aid of small molecules referred to as mediators. Thanks to their diverse nature, laccases have gained attention of both scientists and entrepreneurs from all over the world. Their significance is reflected in countless scientific and industrial applications, wherein laccases have become inseparable from chemical syntheses, the food industry, textile industry, biosensor design and the environmental protection. This paper gathers the most important information and the latest scientific discoveries relating to this desirable biocatalyst.
The efficiency of macrophytes for heavy metals removal from water
(Lodz University of Technology Press, 2017) Materac, Milena; Sobiecka, Elżbieta
Nowadays heavy metals pollution has become one of the most serious environmental problems. Advances in science and technology are leading to the degradation and contamination of aquatic environments. Pollution in aquatic ecosystems poses a serious threat to aquatic biodiversity and serves health hazards in humans. The traditional methods of the environment cleaning in practice are expensive and non-eco friendly can lead to the secondary pollution. It is a reason why the aquatic plants (macrophytes) as the biological remediation methods have been used. Macrophytes play an important role in the biological methods of the water remediation because they have capability to improve the quality of water by absorbing heavy metals with their leaves and effective root systems. This review discusses the potential of different aquatic plants (macrophytes) in purifying water and wastewater.
Antimicrobial effect of lining leather fatliquored with the addition of essential oils
(Wydawnictwo Politechniki Łódzkiej, 2017) Bielak, Elżbieta; Syguła-Cholewińska, Justyna
Footwear, especially those often and intensively used, provides an ideal environment for microbial growth. An appropriate high temperature and moisture content inside it enhance bacterial and fungal colonization by microbes. These microorganisms can be potentially pathogenic to human health and responsible for destruction of shoes materials from which the parts inside the shoe are made. In previous studies it has been shown that the addition of essential oils of antiseptic activity at leather finishing operations allows a leather to get antimicrobial properties. The aim of this paper is to assess the durability of antimicrobial effect of leather fatliquored with the addition of cinnamon, thyme and oregano essential oils at concentration of 5% per leather weight. Antimicrobial activity was evaluated according to guidelines of PN-EN ISO 20645:2006 “Textile fabrics – determination of antibacterial activity – Agar diffusion plate test” after 36-month storage. The obtained results have indicated that leathers under investigations show antimicrobial activity against E. coli, S. aureus and C. albicans strains even 3 years after fatliquoring. The use of natural bioactive substances such as cinnamon, thyme and oregano oils at concentration of 5% per leather weight in the leather finishing process may be an alternative to biocides used in the tanning industry as well it can improve hygienic properties of shoes, internal parts of which are made of such finished leathers.
Elimination of ochratoxin A by lactic acid bacteria strains isolated from chickens and their probiotic characteristics
(Wydawnictwo Politechniki Łódzkiej, 2017) Kupryś-Caruk, Marta; Stefańska, Ilona; Chabłowska, Beata
The aim of the study was to evaluate the ability of lactic acid bacteria strains, isolated from the gastrointestinal tract of chickens, to eliminate ochratoxin A (OTA) in vitro and to investigate whether the mechanism of OTA reduction is adsorption or hydrolysis. The probiotic characteristics of the strains, such as their growth performance and synthesis of lactic acid at 42°C, inhibition of Salmonella spp. and Escherichia spp. growth and susceptibility to antibiotics, was also evaluated. Ochratoxin A reductions in MRS broth depended on the tested strain and ranged from 1% to 29%. The level of OTA reduction was higher at 30°C than at 37 and 42°C and there were no significance differences between OTA reduction obtained with live and heat-treated bacteria cells. The evaluation of probiotic characteristics showed that Pediococcus acidilactici KKP 879 was the strain with more potential to develop a probiotic culture for chickens.
Antioxidative capacity of birch saps
(Lodz University of Technology Press, 2017) Bilek, Maciej; Siembida, Agnieszka; Gostkowski, Michał; Stawarczyk, Kinga; Cieślik, Ewa
In our subsequent studies of birch tree saps we focused on assessing their antioxidant capacity. For research we chose four groups of silver birch trees (Betula pendula Roth.), consisting of five individuals. Antioxidant capacity was examined using spectrophotometric technique. The highest antioxidant capacity, tested by the Folin-Ciocalteau method and expressed as a total phenolics content was 6.59 mg GAE/100 ml of tree sap, and in turn the lowest one 0.88 mg GAE/100 ml. The highest antioxidant capacity determined ABTS method and expressed as radical scavenging activity (RSA) was 30.9% and lowest 5.38% The average values of antioxidant capacity both expressed as a phenolic compounds content and as radical scavenging activity for tree saps collected from four particular locations did not differ significantly. Based on the obtained results of tree sap analyses, it can be claimed that compared to other food products, e.g. fruit and vegetable juices, birch saps are not a rich source of phenolic compounds and when compared e.g. to tee infusions, they have low antioxidant capacity. Although it can be increased by the introduction of functional additives, such as herbal extracts or concentrated fruit juices.
Effect of thermal processing on antioxidant power and thiosulfinate content in Brussels sprouts juices
(Wydawnictwo Politechniki Łódzkiej, 2017) Kot, Mirosława; Olech, Zofia
The aim of this study was to elucidate the influence of thermal processing at 22-95oC on the antioxidant ability of Brussels sprouts juice and its thiosulfinate content. The antioxidant power was determined by FRAP, Folin-Ciocalteu and DPPH radical scavenging methods. Thiosulfinate concentration was assayed by Han's procedure. It was shown that the increase of processing temperature decreased of the antioxidant ability determined by Folin-Ciocalteu method. However, FRAP and DPPH scavenging showed increase by 20% of the antioxidant power in the final heating step (95oC). The increasing processing temperature resulted in decreasing thiosulfinate concentration until the total loss. This proved that the thiosulfinate present in Brussels sprouts juice is thermolabile compound (not resistant for high temperature). The total loss of the thiosulfinate in high temperature did not reflect the change in antioxidant power. This fact indicated that there was no impact of the thiosulfinate on antioxidant abilities of Brussels sprouts juice or the impact was insignificant among the abundance of other antioxidants. The results showed a good correlation between FRAP and DPPH assays of antioxidant power determination in contrast to correlation with the data of Folin-Ciocalteu procedure.
List of Reviewers
(Wydawnictwo Politechniki Łódzkiej, 2017)
Optical properties of (3-(acetamidomethyl)phenyl) boronic acid and its interactions with selected sugars
(Wydawnictwo Politechniki Łódzkiej, 2018) Kur, Katarzyna; Przybyt, Małgorzata; Miller, Ewa
(3-(Acetamidomethyl)phenyl)boronic acid (3AAPBA)has at pH 7 absorbance maximum at 270 nm with molar absorbance coefficient 516 M-1cm-1. 3AAPBA exhibits weak fluorescence with maximum at 297 nm and quantum yield 0.062 ± 0.001. Fluorescence decay is monoexponential and the lifetime is 2.05 ± 0.01 ns. Interactions of 3AAPBA with selected sugars were studied by absorbance, steady-state and time-resolved fluorescence measurements. At pH 7 fluorescence of 3AAPBA is quenched only by fructose (with quenching constant 67.9 M-1) and to some extend by galactose. Addition of these two monosaccharides causes also changes of absorbance spectra of 3AAPBA. Acid-base dissociation of free 3AAPBA and its esters with sugars was studied by absorbance and steady-state fluorescence measurements in pH range from 4.5 to 11.00. Esterification of phenylboronic acid derivatives by sugars leads to increased acidity of them. In case of 3AAPBA the obtained values of pK indicate that affinity of studied sugars towards it can be ordered as follows: fructose > galactose > glucose > maltose > lactose > sucrose. At pHs higher than pK the fluorescence decays turn to biexponential with additional shorter component in lifetime which we propose to attribute to anionic form of 3AAPBA or its esters.
Application and properties of selected flavanones
(Wydawnictwo Politechniki Łódzkiej, 2018) Błazińska, Paulina; Sykuła, Anna
Flavanones, secondary plant metabolites, are one of the main group of flavanoids. They are widely spread in nature in many plants. The large diversity of structural structure of flavanones and controlled methods of modifying their molecules have a huge impact on biological activity. The present review will summarize the current knowledge about occurrence, obtaining by chemical synthesis, application and bioactivity of flavanones. Also, they are received from specific chemical synthesis. Flavanones have a great biological activity. Derivatives of flavanone have many different properties such as anti-inflammatory, anticancer, antioxidant, antimicrobial or hepatoprotective activities. These natural polyphenolic compounds are used in cosmetology, pharmacy and medicine. The demand and usage on them increases.
The DFT study on the electronic structure of boronic acid derivatives and its esters with fructose
(Wydawnictwo Politechniki Łódzkiej, 2018) Kur, Katarzyna; Kowalska-Baron, Agnieszka; Miller, Ewa
Theoretical investigations are carried out to examine the geometrical structure and parameters of electron transitions to the lowest excited states of two boronic acid derivatives: 3-aminophenylboronic acid and 3-(acetamidomethyl)phenyl boronic acid and its cycling esters with fructose, using the DFT based 6-31 G(d,p) method. The most stable ester isomer of each acid has been selected. Predicted excitation wavelength are shorter (less than 0.5 eV) than experimental ones, what is in a good agreement considering limitations of the DFT method. In case of almost every calculated molecule the analysis of electronic transitions shows that transition S0→S1 involves electron transfer mainly from the HOMO to LUMO orbital.
Antioxidant Potential and Phytochemical Content of Selected Fruits and Vegetables Consumed in Cyprus
(Wydawnictwo Politechniki Łódzkiej, 2018) Georgiadou, Egli C; Goulas, Vlasios; Majak, Iwona; Ioannou, Antigoni; Leszczyńska, Joanna; Fotopoulos, Vasileios
Consuming a diet high in fruits and vegetables can lower the risk of developing numerous chronic diseases, including cancer and cardiovascular disease, due to the presence of multiple antioxidants. Horticultural produce consumed in Cyprus include a large variety of fruits and vegetables, most of which are common components of a Mediterranean-style diet due to the island’s localization. The aim of the present study was to evaluate the antioxidant composition and activity of the edible portion of ten fruits and ten vegetables commonly consumed in Cyprus. Total phenolics, anthocyanins, ascorbic acid and carotenoids contents were determined, while the in vitro antioxidant activity was evaluated by three assays. Antioxidant activity showed great variation, with the highest values found in green olives, capers and red chili peppers and in correlation with total phenolic content. Ascorbic acid was detected in large quantities in parsley, coriander, red guavas and red chili peppers, while red chili peppers, capers and coriander had high levels of carotenoids. Furthermore, sweet cherries, red grapes, and red apples contained significant amounts of anthocyanins. Statistical analysis revealed that phenolic compounds are the most potent antioxidants in fruit and vegetables, while total anthocyanins had a weak contribution to their antioxidant activity. The present study could be a guide for Cypriot as well as Mediterranean diet health-conscious consumers to select specific fruits and vegetables as dietary components. To the best of our knowledge, this is the first study thoroughly describing the antioxidant composition and activity of a wide array of important Cypriot horticultural products.
Analysis of the selected antioxidant compounds n ice cream supplemented with Spirulina (Arthrospira platensis) extract
(Wydawnictwo Politechniki Łódzkiej, 2018) Szmejda, Klaudia; Duliński, Robert; Byczyński, Łukasz; Karbowski, Adrian; Florczyk, Tomasz; Żyła, Krzysztof
The research is aimed at evidencing that ice-cream formulations incorporating algae can have health-benefiting effects on human body. The main task of the project is to design ice-cream product line that distinguish itself from regular ice-cream by increased anti-oxidant activity resulted from inclusion of the algae extract. The currently known research evidences that ice-cream can be effective as carriers of health-promoting probiotic bacteria, which in turn encourages also application of other microorganisms in particular algae of specific strains (e.g. Spirulina platensis) as a supplement to ice-cream. In submitted research, the level of polyphenols and antioxidant activity expressed as degree of inhibiting generation of cationo-free radical from DPPH•+ solution were analyzed. Preliminary results based on antioxidative activity tests measured with potential to quench free radicals have shown that ice-cream formulations enriched with algae extract exhibit significantly higher potential achieving inhibition level of 39.7% in the mint ice cream samples as compared to 32.8% inhibition for the control sample without algae. Furthermore, each of the examined samples (dairy, pistachio, mint) ice creams versions supplemented with Spirulina were characterized by enhanced antioxidant activities expressed as potential to quench free radicals and the carotenoids content.