Experimental and theoretical investigation of drotaverine binding to bovine serum albumin

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dc.contributor.authorGałęcki, Krystian
dc.contributor.authorCourtade, Gaston
dc.contributor.authorMcFall, Aisling
dc.contributor.authorPrieschl, Teixeira Renata
dc.contributor.authorGrgic, Maja
dc.contributor.authorEsteve-Sistere, Marta
dc.contributor.authorMaglemose, Westphalen Rikke
dc.contributor.authorKowalska-Baron, Agnieszka
dc.date.accessioned2015-06-03T11:11:23Z
dc.date.available2015-06-03T11:11:23Z
dc.date.issued2013
dc.description.abstractThis study was motivated by the need to provide more insight into the possible mechanism of the intermolecular interactions between antispasmodic drug drotaverine and one of the serum albumins (BSA), with the aim to indicate the most probable sites of these interactions. For this purpose both experimental (spectrofluorometric titration at various temperatures) and theoretical (molecular mechanics) methods have been applied. The obtained results clearly showed that drotaverine quenched BSA fluorescence, and the most probable mechanism is static quenching. The negative value of the theoretically predicted binding free Gibbs energy (-23.8 kJ/mol) confirmed the existence of the intermolecular interactions involving drotaverine and one tryptophan within BSA protein and was well agreed with the experimentally determined value of -25.2 kJ/mol.en_EN
dc.formatapplication/pdf
dc.identifier.citationBiotechnology and Food Science, 2013 Vol.77 nr 1 s.25-36
dc.identifier.issn2084-0136
dc.identifier.other0000043347
dc.identifier.urihttp://www.bfs.p.lodz.pl
dc.language.isoenen_EN
dc.publisherWydawnictwo Politechniki Łódzkiejpl_PL
dc.publisherLodz University of Technology. Pressen_EN
dc.relation.ispartofseriesZeszyty Naukowe Politechniki Łódzkiejpl_PL
dc.relation.ispartofseriesBiotechnology and Food Scienceen_EN
dc.subjectbovine serum albuminen_EN
dc.subjectdrotaverineen_EN
dc.subjectfluorescence quenchingen_EN
dc.subjectphosphorescenceen_EN
dc.subjectmolecular dockingen_EN
dc.subjectalbumina surowicy bydlęcejpl_PL
dc.subjectfosforescencjapl_PL
dc.subjectdrotawerynapl_PL
dc.subjectfluorescencjapl_PL
dc.subjecthartowaniepl_PL
dc.titleExperimental and theoretical investigation of drotaverine binding to bovine serum albuminen_EN
dc.typeArticleen_EN
dc.typeArtykułpl_PL

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